Table 1. Calibration curves for the determination of linearity in the HPLC method. Concentration (μg/mL),Area* (V x sec) ,Day 1,Day 2,Day 3 200,3328.4 ± 51.50,3258.2 ± 36.45,3202.2 ± 25.02 100,1687.95 ± 80.33,1632.19 ± 22.76,1612.5 ± 41.00 50,858.23 ± 40.85,821.71 ± 29.23,807.84 ± 13.53 25,396.28 ± 1.33,394.65 ± 6.65,383.29 ± 7.245 12.5,194.83 ± 8.27,189.15 ± 8.34,191.17 ± 20.87 6.25,107.63 ± 1.20,102.19 ± 0.91,97.11 ± 2.26 3.125,49.52 ± 1.69,47.39 ± 2.75,48.98 ± 2.24 R2,0.9998,1.000,0.9999